• 1. What is the need of identifying autoantibody markers?

 

Reliable predictors of disease —Autoantibody markers provide a disease-specific fingerprint for cancers, autoimmune and other diseases.

 

Available—autoantibodies are highly enriched in easily accessible biological fluids such as serum, plasma, tears, and saliva.

Stable and easy to detect—their long half-lives and sensitive detection strategy provide a simple, robust solution for rapidly detecting autoantibodies.

 

 

2.  How autoantibodies are identified using protein chip technology?

 

Steps

1. Samples are obtained from control and diseased or treated patients.


2.  Protein Microarrays are blocked and incubated with diluted samples. Autoantibody biomarkers are visualized with a fluorescent dye-labeled secondary antibody. IgA, IgG and IgM subtypes can be detected on protein microarrays with the proper secondary antibody.


3. Data analysis with the free software tool protein chip Prospector enables identification of antigens recognized by elevated autoantibodies in a patient’s sample.

 

•